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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
室温
- 英文名：
Wright's Stain Solution
- 库存：
99
- 供应商：
北京绿百草科技发展有限公司
- 规格：
100ML
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文献和实验Procedure 1. After sperm preparation, adjust sperm concentration as high as possible (5-10 x 106 /ml). 2. Prepare a smear of sperm suspention onto a slide and allow it to dry. 3. Place slides in 0.1% (w/v) naphthol yellow S in 1% (v
Testing for Mycoplasma by Indirect DNA Stain (Hoechst 33258 stain)
stain solution (Prod. No. H6024 ) Vero cells (Prod. No. 84113001-1v1) Mountant (Autoclave 22.2ml 0.2M citric acid with 27.8ml 0.2M disodium phosphate. Add 50ml glycerol. Filter sterilize and store at 4ºC) (Prod. No. M1289 ) Mycoplasma
Sensitization: 200ml 0.02% Na2S2O3 1min. Rinse:H2O 2 x 1min. Stain: 200ml chilled 0.1% AgNO3 for minigel (0.15% for large gel) for 20min. Keep from light. Rinse: H2O 2 x 1min. Develop: 2% Na2CO3 + 0.04% formaldehide with intensive shaking. After 20-30
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