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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
4C
- 英文名：
Bullet Blocking One for Western Blotting
- 库存：
1337
- 供应商：
北京绿百草科技发展有限公司
- CAS号：
989-51-5
- 规格：
100MG
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文献和实验room or cool the unit with a refrigerated circulating bath. 3. Immerse the nitrocellulose sheet in chosen blocking solution and incubate for 60 minutes to overnight at RT on a rocking platform. 4. Incubate the sheet in antisera for 90 minutes
After transferring proteins to the membrane, Western blotting procedures require that unreacted binding sites on the membrane be blocked with a non-relevant protein solution. In addition to blocking all remaining binding sites on the membrane, a blocking buffer
Method Determine the highest antibody dilution at which the optimal working dilution is used with the antibody in Western blotting. For neutralization, combine antibody with a fivefold (by weight) excess of blocking peptide in a small
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