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Rhodopsin Antibody
1 mg/ml
WB | IHC | ICC/IF | IP | ELISA
小鼠
哺乳动物 | 鱼 | 凤尾鱼 | 鸟类 | 两栖动物 | 鲨 | 已灭绝的刺鲨 (Acanthodes bridgei)
无标记
多克隆
-20ºC
液体
IgG1
牛视紫红质
12 µg
Product Name | Rhodopsin Antibody |
Description | Mouse Anti-Bovine Rhodopsin Monoclonal IgG1 |
Species Reactivity | Amphibians, Anchovies, Avian, Extinct Spiny Shark (Acanthodes bridgei), Fish, Mammals, Shark |
Applications | WB , IHC , ICC/IF , IP , ELISA |
Antibody Dilution | WB (1:1000), IHC (1000); optimal dilutions for assays should be determined by the user. |
Host Species | Mouse |
Immunogen Species | Bovine |
Immunogen | Bovine Rhodopsin |
Concentration | 1 mg/ml |
Conjugates | Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
|
Storage Buffer | PBS pH 7.4, 50% glycerol, 0.09% sodium azide |
Storage Temperature | -20ºC |
Shipping Temperature | Blue Ice or 4ºC |
Purification | Protein G Purified |
Clonality | Monoclonal |
Clone Number | 4D2 |
Isotype | IgG1 |
Specificity | Detects ~40kDa. Binds specifically to the N-terminus of Rhodopsin. Does not detect Rhodopsin in invertebrates. |
Cite This Product | Mouse Anti-Bovine Rhodopsin Monoclonal, Clone 4D2 (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SMC-176) |
Certificate Of Analysis | 1 µg/ml of SMC-176 was sufficient for detection of rhodopsin in 10 µg of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Alternative Names | OPN2 Antibody, opsd Antibody, opsin 2 Antibody, opsin 2 rod pigment Antibody, opsin2 Antibody, RHO Antibody, RP4 Antibody, MGC138309 Antibody, Retinitis Pigmentosa 4 Antibody |
Research Areas | Cell Signaling, Neuroscience, Neurotransmitter Receptors, Rhodopsin |
Cellular Localization | Membrane |
Accession Number | NP_001014890.1 |
Gene ID | 509933 |
Swiss Prot | P02699 |
Scientific Background | Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin’s activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5). |
References | 1. Molday R.S., Hicks D., and Molday L. (1987) Invest Ophthalmol Vis Sci. 28: 50-61. 2. Ridge K.D., Lee S.S.J., and Abdulaev N.G. (1996) J of Biol Chem. 271: 7860-7867. 3. Matsuyama T., Yamashita T., Imai H. and Shichida Y. (2009) J Biol Chem. Manuscript M109.063875. 4. Feurstein S.E., et al. (2009) Biochemistry. 48(45): 10733-10742. 5. Iannaccone A., et al. (2006) Vision Res. 46(27): 4556-4567. |
Immunohistochemistry analysis of Mouse retina using Rhodopsin Monoclonal Antibody (SMC-176) at a dilution of 1:1000. Other layers of the retina: IS – inner segment; ONL – outer nuclear layer; OPL – outer plexiform layer; INL – inner nuclear layer; IPL – inner plexiform layer; GCL – ganglion cell layer.
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