小鼠脑内皮瘤细胞,bEnd.3 [BEND3]细胞

小鼠脑内皮瘤细胞,bEnd.3 [BEND3]细胞基本信息
资源编号 hzCC337672
资源名称 bEnd.3 [BEND3]
种属 小鼠脑内皮瘤细胞
形态 内皮样
提供形式 P4-5代T25细胞培养瓶
致病类型 内皮瘤
安全等级 2
模式菌株 未知
应用领域 The cells were transformed by infection with the NTKmT retrovirus vector that expresses polyomavirus middle T antigen.
The endothelial nature of these cells was confirmed by the observed expression of von Willebrand factor and uptake of fluorescently labeled low density lipoprotein (LDL).
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鼠脑内皮瘤细胞,bEnd.3 [BEND3]细胞培养方法
培养基 90%高糖DMEM+10%FBS
生长条件 95%空气+5%二氧化碳 37摄氏度
生长特性 贴壁
存储条件 50%高糖DMEM+40%FBS+10%DMSO 液氮
鼠脑内皮瘤细胞,bEnd.3 [BEND3]细胞详细说明
Age 6 weeks
Strain BALB/c
Derivation The cells were transformed by infection with the NTKmT retrovirus vector that expresses polyomavirus middle T antigen.
Antigen Expression ICAM-1 +; VCAM-1 +; MAdCAM-1 +
Genes Expressed 颅
von Willebrand factor,ICAM-1 +; VCAM-1 +; MAdCAM-1 +,The endothelial nature of these cells was confirmed by the observed expression of von Willebrand factor and uptake of fluorescently labeled low density lipoprotein (LDL).,The expression of Peyer's Patch high endothelial receptor for lymphocytes, the mucosal vascular addressin (MAdCAM-1) and E-selectin can be induced on bEnd.
Cellular Products von Willebrand factor
Passage History MAdCAM-1 is expressed on the surface of unstimulated bEnd.3 cells at early passages but not at passages greater than 30.
P-selectin can be induced on bEnd.3 cells by Tumor Necrosis Factor alpha (TNF alpha) at both early and late passages but expression is greater at passages over 30.
Vascular cell adhesion molecule 1 (VCAM-1) is constitutively expressed on the cells at early passages but not at passages over 30.
Restrictions The bEnd.3 cell line is provided for research use only under the following terms1. bEnd.3 cells, or products derived from them, must not be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purpose of sale, or producing for sale, bEnd.3 cells or their products. Commercial interests are the exclusive property of The Max Planck Society. 2. Any proposed commercial use of bEnd.3 cells, or products derived from them, must first be negotiated with Dr. Britta Engelhardt, on behalf of the late Dr. Werner Risau, Max-Planck-Institut fur physiologische und klinische Forschung, W.G. Kerckoff Institut, Abteilung molekulare Zellbiologie, Parkstrasse 1, 61231 Bad Nauheim, Germany. Telephone 49-6032-705203/265; FAX 49-6032-072259; email b.engelhardt@kerckoff.mpg.de3. In all papers reporting any use of bEnd.3 cells, or products derived from them, a direct reference will be made to the original publication (Montesano, et al. Cell 62435-445, 1990).
描述 The endothelial nature of these cells was confirmed by the observed expression of von Willebrand factor and uptake of fluorescently labeled low density lipoprotein (LDL).
The expression of Peyer's Patch high endothelial receptor for lymphocytes, the mucosal vascular addressin (MAdCAM-1) and E-selectin can be induced on bEnd.3 cells by cytokines and lipopolysaccharide (LPS).
This induction by Tumor Necrosis Factor alpha (TNF alpha), interleukin 1 (IL-1) or LPS is concentration and time dependent.
MAdCAM-1 is expressed on the surface of unstimulated bEnd.3 cells at early passages but not at passages greater than 30.
Intracellular adhesion molecule 1 (ICAM-1) is constitutively expressed on the cells, and expression is increased by treatment with LPS, IL-1 and TNF alpha.
Vascular cell adhesion molecule 1 (VCAM-1) is constitutively expressed on the cells at early passages but not at passages over 30.
P-selectin can be induced on bEnd.3 cells by Tumor Necrosis Factor alpha (TNF alpha) at both early and late passages but expression is greater at passages over 30.鼠脑内皮瘤细胞,bEnd.3 [BEND3]细胞Bowes Melanoma 黑色素瘤 品牌ATCC 规格25毫升/株. 货期1-2周
MM96L 黑色素瘤 品牌ATCC 规格25毫升/株. 货期1-2周
B16 黑色素瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
A875 黑色素瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
LLC-MK2 恒河猴肾 品牌ATCC 规格25毫升/株. 货期1-2周
A673 横纹肌癌 品牌ATCC 规格25毫升/株. 货期1-2周
A-204 横纹肌肉瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
HEL 红细胞白血病 (Erythroleukemia) 品牌ATCC 规格25毫升/株. 货期1-2周
Hep-2 喉癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
HEp-2 喉表皮样癌 品牌ATCC 规格25毫升/株. 货期1-2周
RF/6A 猴脉络膜-视网膜细胞(内皮) 品牌ATCC 规格25毫升/株. 货期1-2周
HepII 猴肾 品牌ATCC 规格25毫升/株. 货期1-2周
CV-1 猴肾细胞 品牌ATCC 规格25毫升/株. 货期1-2周
Vero 猴肾细胞 品牌ATCC 规格25毫升/株. 货期1-2周
HCT-8 回盲肠腺癌 品牌ATCC 规格25毫升/株. 货期1-2周
HCT-8 回盲肠腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
L-6TG 肌母细胞 品牌ATCC 规格25毫升/株. 货期1-2周
C6 胶质瘤 品牌ATCC 规格25毫升/株. 货期1-2周
SHG-44 胶质瘤 品牌ATCC 规格25毫升/株. 货期1-2周
Caco-2 结肠癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
CoLo205 结肠癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
CoLo 320DM 结肠癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
Lovo 结肠癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
SW480 结肠癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
LoVo 结肠腺癌 品牌ATCC 规格25毫升/株. 货期1-2周
Ls-174-T 结肠腺癌 品牌ATCC 规格25毫升/株. 货期1-2周
HT-29 结肠腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
HZ-hxbz-057 结直肠癌细胞 LOVE1 规格25毫升/株.
BHK-21 金黄地鼠肾 品牌ATCC 规格25毫升/株. 货期1-2周
Dami 巨核细胞 品牌ATCC 规格25毫升/株. 货期1-2周
KB 口腔表皮样癌 品牌ATCC 规格25毫升/株. 货期1-2周
KB 口腔表皮样癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
SF9 昆虫卵巢细胞 品牌ATCC 规格25毫升/株. 货期1-2周
Sf-21 昆虫细胞系 品牌ATCC 规格25毫升/株. 货期1-2周
SF-9 昆虫细胞系 品牌ATCC 规格25毫升/株. 货期1-2周
L1210 淋巴白血病 品牌ATCC 规格25毫升/株. 货期1-2周
L1210 淋巴白血病细胞 品牌ATCC 规格25毫升/株. 货期1-2周
EL4 淋巴瘤 品牌ATCC 规格25毫升/株. 货期1-2周
EL4IL-2 淋巴瘤 品牌ATCC 规格25毫升/株. 货期1-2周
YAC-1 淋巴瘤 品牌ATCC 规格25毫升/株. 货期1-2周
YAC-1 淋巴瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
U-937 淋巴瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
MOLT-4 淋巴母细胞性白血病细胞系 品牌ATCC 规格25毫升/株. 货期1-2周