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PeproTech重组人/小鼠/大鼠Activin A (C

HO cell derived) 10ug
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  • PeproTech已认证
  • 120-14P
  • 美国
  • 2025年12月07日
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    • 库存：

      大量

    • 供应商：

      PeproTech(派普泰克)

    • 英文名：

      Recombinant Human/Murine/Rat Activin A (CHO cell derived)

    Recombinant Human/Murine/Rat Activin A (CHO cell derived)

    Catalog Number:
    120-14P
    Synonyms:
    Inhibin beta-1, FRP, FSH (Follicle-stimulating hormone)-releasing protein
    Description:
    Activin A is a TGF-β family member that exhibits a wide range of biological activities, including regulation of cellularproliferation and differentiation, and promotion of neuronal survival. Elevated levels of Activin A in human colorectal tumors andin postmenopausal women have been implicated in colorectal and breast cancers, respectively. The biological activities of Activin Acan be neutralized by inhibins and by the diffusible TGF-β antagonist, follistatin. Activin A binds to the two forms of activinreceptor type I (Act RI-A and Act RI-B) and two forms of activin receptor type II (Act RII-A and Act RII-B). Activins arehomodimers or heterodimers of different β subunits. They are produced as precursor proteins with an amino terminal propeptidethat is cleaved to release the C-terminal bioactive ligand. Recombinant Human/Murine/Rat Activin A is a 26.0 kDa disulfide-linked homodimer of two βA chains, each containing 116 amino acid residues.
    AA Sequence:
    GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS
    Source:
    CHO cells
    Purity:
    Greater than 95% by SDS-PAGE gel and HPLC analyses.
    Biological Activity:
    ​Determined by its ability to inhibit the proliferation of mouse MPC-11 cells. The expected ED50 is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.
    Research Interests:
    Country of Origin:
    USA

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    图标文献和实验
    相关实验

    • PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID

      The technique described here is a slight modification (March 1997) of methods presented in: Nagy A., J. Rossant. 1993. Production of completely ES cell-derived fetuses. In : Gene Targeting: A Practical Approach. (ed. A. Joyner). IRL Press at Oxford

    • CHO细胞株的部分特性

      cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. Passages: 17 Karyotype: Hypodiploid, modal No 20Hazard: CYA subclone of the parental CHO cell line, which was derived from the ovary of an adult Chinese hamster. Cells require proline

    • PCR产物克隆(10)

      后都只是平端产物。这种平端克隆往往效率低，并且有非特异性背景菌落产生，造成筛选困难。Topo平端克隆主要改良载体设计，把ccdB （Control of cell death）基因并入到mcs中，如果没有DNA片断插入，ccdB基因则会表达，ccdB基因的表达蛋白，会破坏细菌的DNA gyrase，造成细菌染色体的降解导致细菌死亡。如果有插入片断，则ccdB基因表达受到阻碍，所以细胞可生长。这样可以把高背景的缺点改善，节省筛选的时间。Topo平端克隆与Topo TA方法一样，操作简单快速。Zero

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