基本信息
资源编号 YBCC337870
资源名称 SK-UT-1
种属 人子宫平滑肌瘤细胞
形态 epithelial
提供形式 P4-5代T25细胞培养瓶
SK-UT-1人子宫平滑肌瘤细胞致病类型 grade III,mesodermal tumor (mixed); consistent with leiomyosarcoma
安全等级 2
模式菌株 未知
说明书 点击下载
产品图片 点击下载
培养方法
培养基 90%RPMI-1640+10%FBS
SK-UT-1人子宫平滑肌瘤细胞传代方法 Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 2.0 to 3.0 mL of complete growth medium and aspirate cells by gently pipetting Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:12 is recommended. Medium Renewal: Twice per week
生长条件 95%空气+5%二氧化碳 37摄氏度
生长特性 贴壁生长
存储条件 50% RPMI-1640+40%FBS+10%DMSO 液氮
详细说明
Age 75 years
Gender female
Ethnicity Caucasian
Karyotype (P8) hypodiploid to hyperdiploid
Clinical Data 75 years
Caucasian
female
Antigen Expression Blood Type B; Rh+
Tumorigenic Yes
Effects Yes, in nude mice
STR Profile Amelogenin X
CSF1PO 10,11
D13S317 13
D16S539 13,14
D5S818 10,11
D7S820 9,10
THO1 7
TPOX 8
vWA 15,16
Isoenzymes AK-1, 1
ES-D, 1
G6PD, B
Me-2, 1-2
PGM1, 1
PGM3, 1
Restrictions The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the cells subject to the following 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with the Office of Technology Development, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065. Contact email otd@mskcc.org
SK-UT-1人子宫平滑肌瘤细胞描述 The cells form spindle cell sarcomas.
YB-ATCC-4073 猫肾细胞 进口美国ATCC细胞株,1ml/株
YB-ATCC-4074 中国仓鼠卵巢细胞 进口美国ATCC细胞株,1ml/株
YB-ATCC-4075 非洲绿猴非洲绿猴肾细胞 进口美国ATCC细胞株,1ml/株
YB-ATCC-4076 牛胚气管细胞 进口美国ATCC细胞株,1ml/株
YB-ATCC-4077 恒河猴胚肾细胞 进口美国ATCC细胞株,1ml/株
YB-ATCC-4078 大鼠肝癌细胞 进口美国ATCC细胞株,1ml/株
YB-ATCC-4079 大鼠肝细胞瘤 进口美国ATCC细胞株,1ml/株
YB-ATCC-4080 大鼠心肌细胞 进口美国ATCC细胞株,1ml/株
YB-ATCC-4081 大鼠肾小球系膜细胞EC (HBZY-1) 进口美国ATCC细胞株,1ml/株
YB-ATCC-4082 绿猴恒河猴肾细胞 进口美国ATCC细胞株,1ml/株
YB-ATCC-4083 牛肾细胞 进口美国ATCC细胞株,1ml/株
YB-ATCC-4084 貂肺上皮细胞 进口美国ATCC细胞株,1ml/株