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- 文献和实验
- 技术资料
- 抗体名:
抗 His 标签单克隆抗体 22H1(兔源)
- 抗体英文名:
MonoRab Anti His Tag Antibody 22H1 mAb Rabbit
- 靶点:
His 标签
- 浓度:
0.5mg/mL(常规浓度,以官方说明书为准)
- 应用范围:
ELISA、免疫印迹、免疫组化、免疫沉淀
- 宿主:
兔(Rabbit)
- 适应物种:
通用
- 保质期:
- 20℃可稳定保存 半 年
- 抗原来源:
His 标签合成肽
- 级别:
科研级
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 标记物:
无(Unconjugated)
- 克隆性:
单克隆(Monoclonal)
- 保存条件:
-20℃,可稳定保存 半 年
- 形态:
液体(Liquid)
- 亚型:
Rabbit IgG
- 免疫原:
His 标签合成肽
- 规格:
100μl
| Specificity | Anti-His Tag Antibody (22H1), mAb, Rabbit recognises 6×His epitope tag localized at the C-terminal, N-terminal, and internal region of fusion proteins. It demonstrates high affinity for 10×His, 6×His, and 5×His, with reduced affinity for 4×His and 3×His. Cross-reactivity with yeast lysates is negligible, while slight cross-reactivity is observed with crude E. coli and mammalian lysates. |
| Host Species | Rabbit |
| Immunogen | A synthetic peptide HHHHHH coupled to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Buffer | Supplied in PBS (pH 7.2), containing 10 mg/ml BSA, 0.03% Proclin 300 and 50% glycerol. |
| Storage Instructions | Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed. |
| Purification | Protein A affinity column |
| Isotype | Rabbit IgG, κ |
| Clonality | Monoclonal |
| Clone ID | 22H1 |
| Note | We can customize this product per customer's request including product size, buffer components, etc. |
| Target Background | The His epitope tag, commonly referred to as the His-tag, is a short sequence of histidine residues—typically 6, but sometimes ranging from 4 to 10—added to proteins for purification and detection purposes. Proteins tagged with His residues can be easily captured by a metal-chelating resin and subsequently eluted under mild conditions, preserving protein activity and structure. His-tags can be positioned at either the N- or C-terminus of the protein, and their small size minimizes potential interference with protein function. In addition to purification, His-tags are also valuable in Western blotting, ELISA, and immunoprecipitation, where anti-His antibodies are used for detection. Due to their versatility and simplicity, His-tags have become a standard tool in recombinant protein production and research. |
| Synonyms | His, His Tag, His epitope tag, |
Anti-His Tag Antibody 抗体、抗 His 标签抗体、His 标签抗体、22H1 抗体、兔源抗体、单克隆抗体、标签抗体、WB 抗体、IHC 抗体、IF 抗体、ELISA 抗体
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文献和实验祝贺〖雅裕睿安〗成为Signalway Antibody(SAB)独家
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技术资料









