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- 文献和实验
- 技术资料
- 抗体名:
TRAP 单克隆抗体(兔源)
- 抗体英文名:
TRAP Antibody, mAb, Rabbit
- 靶点:
TRAP
- 浓度:
0.5mg/mL(常规浓度,以官方说明书为准)
- 应用范围:
Western Blot、免疫细胞化学 / 免疫荧光 (ICC/IF)、免疫组化 (IHC)、免疫沉淀 (IP)
- 宿主:
兔(Rabbit)
- 适应物种:
人、小鼠、大鼠
- 保质期:
- 20℃可稳定保存 半 年
- 抗原来源:
人 TRAP 来源合成肽
- 级别:
科研级
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 标记物:
无(Unconjugated)
- 克隆性:
单克隆(Monoclonal)
- 保存条件:
-20℃,可稳定保存 半 年
- 形态:
液体(Liquid)
- 亚型:
Rabbit IgG
- 免疫原:
人 TRAP 来源合成肽
- 规格:
50μl/100μl
| 规格: | 50μl | 产品价格: | ¥1200.0 |
|---|---|---|---|
| 规格: | 100μl | 产品价格: | ¥2200.0 |
| Specificity | This product is specific to TRAP |
| Host Species | Rabbit |
| Immunogen | A synthesized peptide derived from human TRAP |
| Species Reactivity | Human; Mouse; Rat |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Buffer | Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| Storage Instructions | Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed. |
| Purification | Affinity-chromatography |
| Isotype | Rabbit IgG |
| Clonality | Monoclonal |
| Target Background | Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias. |
| Synonyms | TRAP; SPENCDI; TrATPase; |
TRAP 抗体、抗酒石酸酸性磷酸酶抗体、ACP5 抗体、兔源 TRAP 抗体、单克隆 TRAP 抗体、抗 TRAP 抗体、骨相关抗体、破骨细胞相关抗体、WB 抗体、IHC 抗体、IF 抗体、ELISA 抗体
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文献和实验大鼠抗酒石酸酸性磷酸酶 (TRAP)酶联免疫分析(ELISA)
(TRAP) ,再与 HRP 标记的酒石酸酸性磷酸酶抗原结合,形成抗原 - 抗体 - 酶标抗原复合物,经过彻底洗涤后加底物 TMB 显色。 TMB 在 HRP 酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的 抗酒石酸酸性磷酸酶 (TRAP) 呈正相关。用酶标仪在 450nm 波长下测定吸光度( OD 值),通过标准曲线计算样品中 大鼠 抗酒石酸酸性磷酸酶 (TRAP) 浓度。 试剂盒组成 : 试剂盒组成
Interaction Trap/Two‐Hybrid System to Identify Interacting Proteins
method (or interaction trap) is a powerful technique for detecting protein interactions. The procedure is performed using transcriptional activation of a dual reporter system in yeast to identify interactions between a protein of interest (the bait
Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
, which cannot be achieved with conventional antibodies even after 12 h of incubation. Moreover, after precipitation with the GFP-Trap only the antigen (GFP) was detectable on a coomassie gel whereas the typical antibody fragments (light chain, 25 kDa; heavy chain, 50 kDa
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