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Tris-Tricine-SDS PAGE Buffer (

10X)Tris-Tricine SDS-PAGE 电泳缓冲液(10X)
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  • 美国Nationaldiagnostics
  • 美国Nationaldiagnostics
  • 2025年12月20日
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    Tris-Tricine-SDS PAGE Buffer (10X)

    Tris-Tricine SDS-PAGE 电泳缓冲液(10X)
    • Running Buffer for Small Proteins
    • Variation on the Schagger/von Jagow System
    • Substitute for Tris-Glycine-SDS in the Standard Laemmli Protocol
    Catalog Number:SG EC-869
    Discontinuous SDS-PAGE employing Tris-Glycine as the tank buffer resolves proteins down to about 15 kd. However, below this size, the proteins do not destack from the SDS micelles running through the gel with the buffer front. In order to resolve proteins in this size range, the Tris-Tricine system of Schagger and von Jagow (1987) was developed.
    An Alternative to the Schagger and von Jagow System involves running Tris-Tricine Gels using National Diagnostics 10X Tris-Tricine-SDS Buffer (EC-869). With National Diagnostics Tris-Tricine-SDS, you can extend the range of SDS-PAGE to resolve smaller proteins with minimal alteration of protocol.
    To provide this level of convenience, National Diagnostics streamlined the original method of Shaggar and Von Jagow (1987 Anal. Biochem 166) by developing Tris-Tricine-SDS cathode tank buffer to be compatible with the standard Laemmli gel/buffer system. This combination resolves proteins as small as 5kD. The researcher simply substitutes National Diagnostics Tris-Tricine-SDS in the upper (cathode) tank, with no other changes from standard Laemmli protocol to extend the resolution of their gels.

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    图标文献和实验
    相关实验
    • Tricine-SDS-PAGE

      .   Cathode buffer (0.1 M Tris, 0.1 M tricine, 0.1% SDS, pH 8.25) Dissolve 6.055 g Tris base and 8.96 g tricine in a total volume of 500 ml dH2O. Filter the solution through a 0.45 µm filter, add 0.5 g electrophoresis-grade SDS

    • Molecular Weight Determinations Using Polyacrylamide Gel Electrophoresis with Tris-Tricine Buffers

      to these molecules. However, the majority of systems used (e.g., the one described by Laemmli [1 ]) cannot separate polypeptides with masses below about 15 kDa. Various methods have been described to extend the range of SDS-PAGE

    • Tricine-SDS-PAGE胶的使用方法

      Tricine胶比较容易将样品压平,具体原因我也没有搞清楚,但可以肯定的是这种胶跑出来的Marker又细又直,同样,显出来的条带也压的很好。与普通的SDS-PAGE胶跑出来的结果相比,Marker扩散没有那么厉害,各泳道条带间隔明显,不像普通胶的条带容易连到一起。反正跑出来的条带就是很漂亮。 Tricine-SDS-PAGE胶的配方:(我一直用的就是10%这个浓度,把8KD

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