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Denaturation Solution DNA/RNA变性液
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文献和实验Alkali Denaturation of Supercoiled Plasmid DNA
实验步骤 1. To 20 ul of purified, concentrated DNA solution add 2 ul of freshly prepared NaOH/EDTA solution and incubate at 37 degrees for 30 minutes. 2. Neutralize the reaction by adding : 3 ul 3M NaOAc 5.2 and 7 ul H2
Targeted Exon Sequencing by In‐Solution Hybrid Selection
a protocol for the targeted enrichment of exons from randomly sheared genomic DNA libraries using an in?solution hybrid selection approach for sequencing on an Illumina Genome Analyzer II. The steps for designing and ordering a hybrid selection oligo pool
PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
of amplification, as the mean length of target DNA is decreased: for templates of 300bp or less, denaturation temperature may be reduced to as low as 88℃ for 50% （G+C） templates （Yap and McGee, 1991）， which means one may do as many as 40 cycles
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