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99
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欣博盛生物
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文献和实验to replace combinattons of leucme coupled with valine in pepttde sequences. The preparation described is the final method developed, after many early less successful attempts. The synthesis mvolves (Fig. 1 ) the coupling of 1-bromo-5-methyl-2-hexene 3 and N
-derived cells were released by teasing them into RPMI-1640 medium supplemented with 20 mM N-[2-hydroxyethyl]piperazine-N′-[2-ethanesulfonic acid] (HEPES) buffer. The splenocytes or bone marrow-derived cells (5 × 106 cells/mL) were cultured in 96
Using the Structure‐Function Linkage Database to Characterize Functional Domains in Enzymes
with 4‐carboxymuconolactone‐decarboxylating and 3‐oxoadipate enol‐lactone‐hydrolyzing activity. J. Bacteriol. 180:1072‐1081. Gerlt, J.A. and Babbitt, P.C. 2001. Divergent evolution of enzymatic
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