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文献和实验Assembly of Nucleosomal Templates by Salt Dialysis
Because it is convenient to assemble nucleosomal templates through salt dialysis, large amounts of chromatin complexes can be made easily and in a short amount of time. This unit includes instructions for the various salt dialysis schemes (step versus gradient
Assaying Different Types of Plant Phospholipase D Activities In Vitro
Over the past decade, tremendous progress has been made toward understanding the physiological functions of individual members of the diverse phospholipase D (PLD) family of enzymes in plants. For instance, the involvement of plant PLD
7?deazapurine (pyrrolo[2,3?d ]pyrimidine) 2??deoxyribonucleosides are synthesized by stereoselective nucleobase anion glycosylation. The introduction of a halogen at C7 is performed regioselectively either on the nucleobase or on the nucleoside. The pK a values
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