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文献和实验Assay of Deoxyhypusine Synthase Activity
Deoxyhypusine synthase catalyzes an unusual protein modification reaction. A portion of spermidine is covalently added to one specific lysine residue of one eukaryotic protein, eIF5A (eukaryotic initiation factor 5A) to form a deoxyhypusine
Measurement of the Nitric Oxide Synthase Activity Using the Citrulline Assay
The free-radical gas nitric oxide (NO) recently has been identified as an important biological messenger molecule in both the central and peripheral nervous system. NO is generated by the enzyme NO synthase (NOS
A Fluorescence-Based Assay for Core 1 3Galactosyltransferase (T-Synthase) Activity
has shown the importance of O-glycans and the absolute requirement of T-synthase in this pathway. The assessment of the T-synthase activity has historically been conducted using a radioactive method. Here we describe a fluorescence-based assay procedure
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