- ￥1700 - 4000
- GeneTex
- 美国
- GTX628546
- 2025年07月11日
- WB, IHC-P
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human Cyclin B1. The exact sequence is proprietary.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX628546
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, IHC-P
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Cyclin B1
- 抗体英文名：
Cyclin B1 antibody [GT238]
- 抗体名：
Cyclin B1 抗体 [GT238]
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Cyclin B1 antibody [GT238] detects Cyclin B1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Cyclin B1 stained by Cyclin B1 antibody [GT238] (GTX628546) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Cyclin B1 antibody [GT238] detects CCNB1 protein by Western blot analysis.
A. 30 μg U2OS whole cell lysate/extract (untreated)
B. 30 μg U2OS whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr)
10 % SDS-PAGE
Cyclin B1 antibody [GT238] (GTX628546) dilution: 1:1000
Cyclin B1 antibody [GT238] detects CCNB1 protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (untreated)
B. 30 μg HeLa whole cell lysate/extract (serum starvation for 48hr)
C. 30 μg HeLa whole cell lysate/extract (2mM thymidine treatment and recovery for 3.5hr)
D. 30 μg HeLa whole cell lysate/extract (2mM thymidine treatment and recovery for 12hr)
10 % SDS-PAGE
Cyclin B1 antibody [GT238] (GTX628546) dilution: 1:500
Cyclin B1 antibody [GT238] detects Cyclin B1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Cyclin B1 stained by Cyclin B1 antibody [GT238] (GTX628546) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献和实验Ming-YangLee et al., European Journal of Pharmacology 2018 839()
of microinjecting cRNA to proteins of interest that have been coupled to a fluorescent protein using cyclin B1 as an example.
IMMUNOSCREENING OF LAMBDA-GT11 BACTERIOPHAGE LIBRARIES WITH ANTIBODY PROBES
one 132 mm nitrocellulose membrane per Petri dish in 10mM IPTG (MW=238.31). Briefly blot the excess solution from the filters, and wrap them in cellophane. These should be moist and stored at 4°C. We typically adsorb serum with five 150 mm Petri
Antibody Screening of Bacteriophage gt11 DNA Expression Libraries
Much of our current understanding of the molecular details of the activity and interactions of proteins has stemmed from the ability to isolate cDNA encoding these proteins. Computer-based analysis of deduced amino acid sequence allows
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