- ￥5000
- GeneTex
- 美国
- GTX60851
- 2025年07月15日
- WB, ChIP assay
- Rabbit
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
TIP5 (Transcription termination factor I-interacting protein 5), using the recombinant protein.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX60851
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ChIP assay
- 靶点：
TIP-5
- 抗体英文名：
TIP-5 antibody - ChIP grade
- 抗体名：
TIP-5 抗体 - ChIP grade
- 规格：
50 μl
ChIP analysis of HEK293T and NIH3T3 cells using GTX60851 TIP-5 antibody - ChIP grade. Chromatin from HEK293T and NIH3T3 cells was formaldehyde cross-linked and sheared with the Bioruptor to yield fragments with an average length of 200 to 400 bp. ChIP was performed overnight at 4˚C with 100 μg sheared chromatin and either 5 μl of the TIP5 antibody or 5 μl IgG which was used as negative IP control. The IP'd DNA was analysed by qPCR with primer sets for the promoter and the coding region of the 28s ribosomal RNA gene. This figure shows the recovery by the TIP5 antibody and by IgG (set to 1), normalised to the input DNA. These results show that, both in HEK293T and in NIH3T3 cells, TIP5 is associated with the promoter, but not with the coding region of the 28srRNA gene.
WB analysis of 150 μg nuclear extract from either NIH3T3 or HeLa cells using GTX60851 TIP-5 antibody - ChIP grade.
Dilution : 1:1,000
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文献和实验Herring Sperm DNA37% Formaldehyde (ACS reagent grade)1.25 M glycineProtocol: (Generalized for all cell types- inquire for details regarding particular cell types) For all the following steps,use the pipets that are specifically designated for ChIP use
CHROMATIN IMMUNOPRECIPITATION (CHIP) PROTOCOL FOR YEAST This protocol is derived from a paper by Miriam Braunstein and is based on work in the Allis lab. The procedure was written by Pam Meluh and updated by Paul Megee (6/10/00).
(700 to 1000 rpm at 4�C, ~1min). Carefully remove the supernatant that contains unbound, non-specific DNA. Wash the protein A agarose/antibody/histone complex for 3-5 minutes on a rotating platform with 1ml of each of the buffers listed in the order
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