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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
请参考说明书
- 亚型:
请参考说明书
- 形态:
液体
- 保存条件:
Aliquot and store at -20ºC
- 克隆性:
请参考说明书
- 标记物:
请参考说明书
- 适应物种:
human/mouse/rat等
- 宿主:
rabbit/mouse
- 应用范围:
ELISA,WB,IHC等
- 浓度:
请参考说明书
- 靶点:
请参考说明书
- 抗体英文名:
Histone H3S10ph (phospho Ser10) antibody - ChIP grade
- 抗体名:
Histone H3S10ph (phospho Ser10) antibody - ChIP grade
- 规格:
50μg
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文献和实验Chromatin Immunoprecipitation (ChIP)
of formaldehyde in the ChIP method was pioneered by Solomon and Varshavsky (Solomon et al. 1988). In their original studies, the association of histone H4 and RNA polymerase II with the Drosophila hsp70 genes was monitored. Cells were treated with formaldehyde
H20 ChIP elution buffer 20ml final volume: 500µl 2M Tris 2ml 10% SDS 400µl 0.5M EDTA 17.1ml H2O cold lysis buffer 1mM PMSF 20units RNasin 10mM Tris-HCl pH 7.4 10mM NaCl 3mM MgCl2
. 45. Bring the volume to 1 ml with ChIP incubation buffer. 46. Add 5-10 µg of the antibody of choice. In order to control for nonspecific background signal, it is important to include a control precipitation with an (IgG
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