- ￥5800
- GeneTex
- 美国
- GTX60578
- 2025年07月08日
- WB, FACS, ELISA
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Purified recombinant fragment of human CEBPA expressed in E. Coli.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX60578
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, FACS, ELISA
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
C/EBP alpha
- 抗体英文名：
C/EBP alpha antibody [4E10]
- 抗体名：
C/EBP alpha 抗体 [4E10]
- 规格：
100 μg
ELISA analysis of antigen using GTX60578 C/EBP alpha antibody [4E10].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
FACS analysis of HeLa cells using GTX60578 C/EBP alpha antibody [4E10].
Green : C/EBP alpha
Red : negative control
WB analysis of THP-1 cell lysate using GTX60578 C/EBP alpha antibody [4E10].
WB analysis of human C/EBP alpha (AA: 139-204) recombinant protein using GTX60578 C/EBP alpha antibody [4E10].
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文献和实验In situ hybridisation to alpha satellite sequences (chromosome specific)
can be done without formamide at higher temperature (try 0.25 x SSC at 72C for 5 minutes) Formamide wash solution: 60ml formamide TOXIC (Sigma F7503), 30ml H2 O, 10ml 20 x SSC, 80 m l conc. HCl (to bring the pH to 7) Do not allow the slides to dry at any stage
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
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