Histone H3 containing the monomethylated lysine 9 (H3K9me1), using a KLH-conjugated synthetic peptide.
亚型:
IgG
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Polyclonal
标记物:
Unconjugated
适应物种:
Human, Nematode
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX60352
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Rabbit
应用范围:
WB, Dot, ELISA, ChIP assay
浓度:
1.17 mg/ml (Please refer to the vial label for the specific concentration.)
ELISA analysis of peptide containing the histone modification of interest using GTX60352 Histone H3K9me1 (Mono-methyl Lys9) antibody - ChIP grade.
ChIP analysis of sheared chromatin from 10⁴ HeLa cells using GTX60352 Histone H3K9me1 (Mono-methyl Lys9) antibody - ChIP grade. Respectively 1 and 5 μg of the antibody and 5 μg of IgG (negative IP control) were used per ChIP experiment. QPCR was performed with primers for the GAPDH promoter and for the inactive gene MYOD. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K9me1 is preferably present at silent genes.
WB analysis of histone extracts (15 μg) from HeLa cells using GTX60352 Histone H3K9me1 (Mono-methyl Lys9) antibody - ChIP grade. Dilution : 1:1,000
Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications and the unmodified sequence of histone H3 using GTX60352 Histone H3K9me1 (Mono-methyl Lys9) antibody - ChIP grade. Dilution : 1:20,000