Histone H3 containing the monomethylated lysine 27 (H3K27me1), using a KLH-conjugated synthetic peptide.
亚型:
IgG
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Polyclonal
标记物:
Unconjugated
适应物种:
Human, Arabidopsis thaliana
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX60350
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Rabbit
应用范围:
WB, ICC/IF, Dot, ELISA, ChIP assay
浓度:
1.93 mg/ml (Please refer to the vial label for the specific concentration.)
ICC/IF analysis of 4% PFA fixed U2OS cells using GTX60350 Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade. Staining of the cells with the H3K27me1 antibody after incubation of the antibody with 2 ng/μl blocking peptide containing the unmodified and the mono-, di- and trimethylated H3K27 (Figure B, C, D, respectively). Red : Primary antibody Blue : DAPI Dilution : 1:1000
ELISA analysis of peptide containing the histone modification of interest using GTX60350 Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade.
ChIP analysis of sheared chromatin from 5x10⁵ K562 cells using GTX60350 Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade. A titration of the antibody consisting of 0.5, 1, 2 and 5 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Figure 1A. Quantitative PCR was performed with primers for the coding sequence of the active GAPDH and ACTB genes, used as positive controls, and for the inactive MYOD1 and MYT1 genes, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). Figure 1B. Recovery of the nucleosomes carrying the H3K27me1, H3K27me2, H3K27me3, H3K4me1, H3K9me1, H3K36me1, H4K20me1 and the unmodified H3K4 as determined by qPCR. The figure clearly shows the antibody is specific in ChIP for the H3K27me1 modification with some slight cross reaction with H3K36me1 and H3K9me1 at higher concentrations.
Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications and unmodified sequences of histone H3 and H4 using GTX60350 Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade. Dilution : 1:20,000
WB analysis of histone extracts (15 μg) from HeLa cells using GTX60350 Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade. Dilution : 1:1,000