- ￥5300
- GeneTex
- 美国
- GTX54748
- 2025年07月11日
- WB, ICC/IF
- Mouse
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Full lengthhuman EIF1 protein produced in HEK293T cells.
- 亚型：
IgG2a
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX54748
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
eIF1
- 抗体英文名：
eIF1 antibody [2B9]
- 抗体名：
eIF1 抗体 [2B9]
- 规格：
100 μg
ICC/IF analysis of HeLa cells and NIH3T3 cells using GTX54748 eIF1 antibody [2B9].
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature
ICC/IF analysis of MCF-7 cells using GTX54748 eIF1 antibody [2B9]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC
WB analysis of 80μg whole cell lysate using GTX54748 eIF1 antibody [2B9].
Dilution : 1:1000
ICC/IF analysis of NIH-3T3 cells using GTX54748 eIF1 antibody [2B9]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC
ICC/IF analysis of HeLa cells using GTX54748 eIF1 antibody [2B9]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC
ICC/IF analysis of MCF-7 cells using GTX54748 eIF1 antibody [2B9]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% PFA
Permeabilization : 0.1% Triton„ X-100 for 10 minutes
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature
WB analysis of whole cell extracts (30 μg lysate) of A549 (Lane 1), Hep G2 (lane 2) and PANC-1 (Lane 3) using GTX54748 eIF1 antibody [2B9].
Dilution : 1:500-1:2000
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文献和实验Pisareva VP et al., Nucleic Acids Res 2016 (PMID:27067542)
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