Purified internal fragment of human recombinant PAX7 expressed in E. coli.
亚型:
IgG
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
克隆性:
Polyclonal
标记物:
Unconjugated
适应物种:
Human, Mouse
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX54533
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Rabbit
应用范围:
WB, ICC/IF, IP
浓度:
1 mg/ml(Please refer to the vial label for the specific concentration.)
靶点:
PAX7
抗体英文名:
PAX7 antibody
抗体名:
PAX7 抗体
规格:
100 μg
ICC/IF analysis of MCF7 and HeLa cells using GTX54533 PAX7 antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:100 for at least 1 hour at room temperature
WB analysis of 50μg of HeLa lysate overexpressing PAX7 (right lane) or empty vector control (left lane) using GTX54533 PAX7 antibody. Dilution : 1:1000
IP analysis of HeLa cell lysates using GTX54533 PAX7 antibody. IP reaction : 3μg antibody / 500μg lysate Dilution : 1:1000
ICC/IF analysis of C2C12 cells using GTX54533 PAX7 antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 and incubated overnight at 4ºC
WB analysis of 50 μg of various whole cell lysates using GTX54533 PAX7 antibody. Dilution : 1:1000
ICC/IF analysis of HeLa cells using GTX54533 PAX7 antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 and incubated overnight at 4ºC
ICC/IF analysis of MCF-7 cells using GTX54533 PAX7 antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 and incubated overnight at 4ºC