- ￥4900
- GeneTex
- 美国
- GTX38594
- 2025年07月11日
- WB, IHC-P
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Peptide sequence around phosphorylation site of threonine 187 (E-Q-T(p)-P-K) derived from human p27 Kip1.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX38594
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IHC-P
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
p27 Kip1 (phospho Thr187)
- 抗体英文名：
p27 Kip1 (phospho Thr187) antibody
- 抗体名：
p27 Kip1 (phospho Thr187) 抗体
- 规格：
50 μl
WB analysis of extracts from HeLa cells untreated or treated with EGF IFN-a using GTX38594 p27 Kip1 (phospho Thr187) antibody.
IHC-P analysis of human breast carcinoma tissue using GTX38594 p27 Kip1 (phospho Thr187) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide
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文献和实验Procedure Dilute p27 monoclonal antibody 1:1000 (v/v) in Carbonate Coating Buffer. Add 100 µl/well and incubate o/n @ 4°C or 1 hr @37°C. Wash 3X with TBST (pour onto plate, empty into sink, hit onto towel 3x to clear wells
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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