- ￥5600
- GeneTex
- 美国
- GTX17353
- 2025年07月16日
- WB, IHC-P
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S10 of human p27Kip1.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX17353
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IHC-P
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
p27 Kip1 (phospho Ser10)
- 抗体英文名：
p27 Kip1 (phospho Ser10) antibody
- 抗体名：
p27 Kip1 (phospho Ser10) 抗体
- 规格：
400 μl
IHC-P analysis of human skeletal muscle using GTX17353 p27 Kip1 (phospho Ser10) antibody.
WB analysis of HL-60 cell lysate using GTX17353 p27 Kip1 (phospho Ser10) antibody.
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文献和实验So EC et al., Onco Targets Ther 2016 (PMID:27175086)
Procedure Dilute p27 monoclonal antibody 1:1000 (v/v) in Carbonate Coating Buffer. Add 100 µl/well and incubate o/n @ 4°C or 1 hr @37°C. Wash 3X with TBST (pour onto plate, empty into sink, hit onto towel 3x to clear wells
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Mouse p27 PCR Using Gitschier Buffer
: 1 M Tris pH 8.8 (do not use pH meter, store at room temp.) 1.23 g Tris HCl 5.13 g Tris base q.s. 50 mL with H2 O KG-1 (10x) (Store frozen) Amt [final] 8.3 mL 1M (NH
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