- ￥5300
- GeneTex
- 美国
- GTX15794
- 2025年07月11日
- WB, ICC/IF, IHC-P, IP, IHC
- Mouse
- Human, Mouse, Rat, Primate
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein containing residues A A T R P D Y I S W G T Q E Q of human gamma Tubulin.
- 亚型：
IgG2b
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Primate
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX15794
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, IP, IHC
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
gamma Tubulin
- 抗体英文名：
gamma Tubulin antibody [4D11]
- 抗体名：
gamma Tubulin 抗体 [4D11]
- 规格：
100 μg
WB analysis of 50 ug of various whole cell lysates using GTX15794 gamma Tubulin antibody [4D11].
Dilution : 1:1000
WB analysis of membrane enriched extracts (30 ug lysate) of A-549 (Lane 1), MCF-7 (Lane 2), HeLa (Lane 3), HepG2 (Lane 4), Caco2 (Lane 5), A-431 (Lane 6), SH-SY5Y (Lane 7) and U-2 OS (Lane 8) using GTX15794 gamma Tubulin antibody [4D11].
Dilution : 1 μg/ml
ICC/IF analysis of HeLa (A) and U2-OS cells (B) using GTX15794 gamma Tubulin antibody [4D11].
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature
IHC-P analysis of human breast carcinoma tissue using GTX15794 gamma Tubulin antibody [4D11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20
WB analysis of gamma tubulin knockdown HeLa cells (lane 3), scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1) using GTX15794 gamma Tubulin antibody [4D11].
Dilution : 1:1000
IHC-P analysis of human colon tissue using GTX15794 gamma Tubulin antibody [4D11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200
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文献和实验Immunofluorescent Localization of Tubulin
antibody to tubulin) FITC-labeled goat anti-rabbit (or secondary antibody to match the primary) Fluorescence Mounting Media Fluorescent Microscope equipped with 490 nm excitation filter and 515 nmbarrier filter Kodachrome film
Immunofluorescent Localization of Tubulin
Rabbit anti-tubulin (or other primary antibody to tubulin) FITC-labeled goat anti-rabbit (or secondary antibody to match the primary) Fluorescence Mounting Media Fluorescent Microscope equipped with 490 nm excitation filter
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
and sensitive to changes in both the chemical and mechanical environment. Optimal conditions for fluorescence of proteins of the actin and tubulin cytoskeleton are based on: preserving cell structure; properties of individual cytoskeletal proteins
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