- ￥4700
- GeneTex
- 美国
- GTX15784
- 2025年07月16日
- WB, ICC/IF, ELISA
- Mouse
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human MCP-1
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX15784
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, ELISA
- 浓度：
1.05 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
MCP1 / CCL2
- 抗体英文名：
MCP1 / CCL2 antibody [E10051]
- 抗体名：
MCP1 / CCL2 抗体 [E10051]
- 规格：
250 μg
ICC/IF analysis of HCT116 cells using GTX15784 MCP1 / CCL2 antibody [E10051]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% PFA
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature
WB analysis of tissue extracts (30 μg lysate) of Mouse Pancreas (Lane 1), Spleen (Lane 2), Lung (Lane 3) and Cerebellum (Lane 4) using GTX15784 MCP1 / CCL2 antibody [E10051].
Dilution : 1-2 μg/ml
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
throughout the procedure. d) Plate the entire fusion out in just 120 x 1ml cultures in 6 x 'Linbro' 24 well plates, using DMEM + 20% FCS (containing penniclin + streptomicin, Na pyruvate and glutamine). e) DO NOT use Isoves medium (IMDM) as this will encourage
E L I S A w i t h p l a t e l e t s
prepare platelets from control and immunized mice distribute 2.5 x106 platelets per well on F-bottomed MaxiSorp Nunc-Immuno microplates (GibcoBRL, Roskilde, Denmark), centrifuge at 3000 rpm, 7 min, 10ѓC. fix with PBS-PF
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
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