- ￥4700
- GeneTex
- 美国
- GTX15760
- 2025年07月15日
- WB, ELISA, IHC, Neutralizing/Inhibition
- Rat
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human IL-4 (CHO cell-derived)
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX15760
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rat
- 应用范围：
WB, ELISA, IHC, Neutralizing/Inhibition
- 浓度：
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
IL4
- 抗体英文名：
IL4 antibody [25D2]
- 抗体名：
IL4 抗体 [25D2]
- 规格：
250 μg
ELISA analysis of 1 μg/mL human IL-4 recombinant protein diluted in carbonate/bicarbonate buffer using serial diluted GTX15759 IL4 antibody [25D2] (Biotin).
WB analysis of 1 ug of human recombiannt IL-4 protein in non-reducing sample buffer using GTX15760 IL4 antibody [25D2] (Biotin).
Dilution : 1:1000
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文献和实验Dimitrova P et al., J Immunol. 2002 (PMID:12218161)
Identification of T-Cell Epitopes
, which secrete γ-interferon, resulting in macrophage activation, or TH2 cells, which secrete interleukin-4 (IL4), resulting in B-cell differentiation and antibody secretion (7 , 8 ). Experiments on T-cells recognizing Leishmania antigens were important
Protocol for intracytoplasmic staining of cytokines for FACS analysis
at 37 deg C. Harvest cells and wash into PBS + 0.1% BSA + 0.1% NaN3. 4) Perform any antibody staining of the cell surface that is sensitive to fixation at this stage. If you are using cells with high levels of Fc receptors, and entirely non
ELISPOT (Enzyme-linked ImmunoSPOT)
of cytokine-producing cells at the single cell level. The method employs the sandwich assay approach of the enzyme-linked immunosorbent assay (ELISA), with some variations. The ELISPOT capture antibody is coated aseptically onto a polyvinylidene difluoride
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