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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Human plasma fibonectin
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX15737
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IP, ELISA, RIA
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Fibronectin
- 抗体英文名:
Fibronectin antibody [3F12]
- 抗体名:
Fibronectin 抗体 [3F12]
- 规格:
100 μg
WB analysis of 25 ug of human plasma lysates using GTX15737 Fibronectin antibody [3F12].
Dilution : 1:300
ICC/IF analysis of HepG2 cells using GTX15737 Fibronectin antibody [3F12]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 overnight at 4 ºC
WB analysis of 30 μg of the Caco-2 whole cell lysates using GTX15737 Fibronectin antibody [3F12].
Dilution : 2 μg/ml
Sandwich ELISA analysis of recombinant human plasma fibronectin protein ranging from 1.6-1000ng/ml, 100ul) using GTX15737 Fibronectin antibody [3F12] (detection antibody) at 1 μg/mL and fibronectin polyclonal antibody (capture antibody).
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文献和实验Cytotoxic Tumor Targeting With scFv Antibody-Modified Liposomes
molecules on the liposome surface are maleimides that are linked to phospholipids composing the liposome bilayer membrane. We illustrate the coupling method of a functionalized single-chain antibody fragment with binding specificity to ED-B fibronectin
spreading, we developed a method engaging integrin signaling by use of an immobilized anti-integrin monoclonal antibody (mab) directed against the fibronectin (FN) receptor integrin α5β1. ECV 304 cells were plated onto FN or immobilized mab JBS5 (anti
by centrifugation. Cell culture. 1. For tissue culture, the collected cells were dispersed with DMEM/Ham F12 containing 10% FCS, 5% chicken embryo extract, and 5% rat serum, as well as 2 mM glutamine, penicillin, and streptomycin. 2.
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