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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原:
The immunogen used to generate this antibody corresponds to Protein A
- 亚型:
IgY
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 保质期:
12 months from the shipping date of the product.
- 目录编号:
GTX127324
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Chicken
- 应用范围:
WB
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Protein A
- 抗体英文名:
Protein A antibody
- 抗体名:
Protein A 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Western blot analysis of 0.1 ng protein A, using protein A antibody(GTX127324) (A) and GFP antibody(GTX124117) (B as negative control). After incubation of primary antibody, Easy blocker (GTX425858) was used to block interaction of protein A with secondary antibody.
A: 1 mg/ml IgY of Protein A (1:10000).
B: 1 mg/ml IgY of GFP (1:10000).
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- 内容
- 询问日期
文献和实验Protein A Purification of Antibody
1. Reagents(1) Affi-gel Protein-A Agarose (BioRad #153-6153)(2) MAPS II Binding Buffer (BioRad # 153-6161)(3) 0.314 g/ml diH2O(4) MAPS II Elution Buffer (BioRad #153-6162)(5) 0.023 g/ml diH2O(6) MAPS II Regeneration Buffer (BioRad #153-6166(7) 1M
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
Protein A, derived from the cell wall of Staphylococcus aureus , and protein G, derived from the cell wall of certain strains of b -hemolytic Streptococcci , binds the Fc region
Antibody Purification: Affinity Chromatography Protein A and Protein G Sepharose
Affinity chromatography relies on the reversible interaction between a protein and a specific ligand immobilized in a chromatographic matrix. The sample is applied under conditions that favor specific binding to the ligand as the result
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