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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein of Influenza A virus NS1 (nonstructural protein) (A/WSN/1933(H1N1). The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Influenza A virus
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Influenza A virus
- 目录编号:
GTX125990
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P
- 浓度:
0.44 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Influenza A virus NS1 (nonstructural protein)
- 抗体英文名:
Influenza A virus NS1 (nonstructural protein) antibody
- 抗体名:
Influenza A virus NS1 (nonstructural protein) 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Influenza A viral lysate and Influenza B viral lysate (0.1 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A virus NS1 (nonstructural protein) antibody (GTX125990) diluted at 1:440. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A virus NS1 (nonstructural protein) antibody (GTX125990) diluted at 1:440. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Influenza A virus NS1 (nonstructural protein) antibody detects Influenza A virus NS1 (nonstructural protein) protein by western blot analysis. Non-infected (-) and Influenza A virus (H1N1)-infected (+) H1299 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A virus NS1 (nonstructural protein) antibody (GTX125990) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sample (10 μg of whole cell lysate)
A: mock DF-1 cell lysates
B: WSN-infected DF-1 cell lysates
12% SDS PAGE
GTX125990 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Influenza A Virus NS1 antibody detects NS1 (H1N1) protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% PFA at RT for 20 min.
Green: NS1 (H1N1) protein stained by Influenza A Virus NS1 antibody (GTX125990) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.
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文献和实验Russell AB et al., Elife 2018 (PMID:29451492)
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Expression of the Hemagglutinin Protein of Influenza Virus: Analysis of Protein Modifications
the capacity of this system to direct the complex and posttranslational modifications involved in the biosynthesis of most membrane and secretory proteins. To probe this question, the influenza virus hemagglutinin was expressed in Spodupteru frugiperdu (Sf
This chapter describes some commonly used methods of influenza virus titration, antigenic characterization, and serological methods by antibody detection. These methods are essential not only for virus characterization
An Immunoproteomics Approach to Screen the Antigenicity of the Influenza Virus
The structure and antigenicity of protein antigens of the influenza virus are screened in a single step employing an immunoproteomics approach. Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) coupled to gel
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