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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthetic peptide corresponding to a region within amino acids 410 and 419 of human c-Myc
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 保质期:
12 months from the shipping date of the product.
- 目录编号:
GTX115046
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IP
- 浓度:
0.54 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Myc tag
- 抗体英文名:
Myc tag antibody
- 抗体名:
Myc tag 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Myc antibody Immunoprecipitation of Myc tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-Myc antibody (GTX115046). (A) Input, 15ug of HeLa transfected lysates. The precipitated protein was detected by GTX115046 diluted at 1:10000. EasyBlot anti-Rabbit IgG Kit (GTX225856-01) was used in Western blot.
Immunofluorescence analysis of PFA-fixed Myc tag transfected HeLa, using Myc tag(GTX115046) antibody (green) at 1:3000 dilution. Alpha-tubulin filaments were labeled with GTX11304 (red) at 1:2500.
Myc tag antibody detects Myc tag protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% PFA at RT for 15 min.
Green: Myc-tag tagged protein stained by Myc tag antibody (GTX115046) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Myc tag antibody (GTX115046) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Aartjan te Velthuis et al., bioRxiv 2018 (Epub)
Healy MD et al., Cell 2023 (PMID:37172566)
Han-Yu Wang et al., Cells 2021 (PMID:33572403)
Satoh Y et al., J Gen Virol 2021 (PMID:34643483)
Kuge H et al., J Lipid Res 2020 (PMID:32963038)
Liu YH et al., Biosci Rep 2020 (PMID:32744317)
Lai HJ et al., J Neurol Sci 2020 (PMID:32247966)
Te Velthuis AJW et al., Nat Microbiol 2018 (PMID:30224800)
Cao C et al., Front Microbiol 2019 (PMID:31969874)
Montes-Chinea NI et al., Neurol Genet 2018 (PMID:30533528)
Thomas S et al., Hum Mutat 2014 (PMID:24166846)
von Morgen P et al., Oncogene 2017 (PMID:28436950)
Guo YC et al., Neurology 2014 (PMID:25253745)
Shen YR et al., PLoS Genet 2017 (PMID:28346465)
Chang KH et al., J Formos Med Assoc 2016 (PMID:27423549)
Wong MT et al., J Virol 2016 (PMID:27489281)
Yen CS et al., Sci Rep 2016 (PMID:27122225)
Ding Y et al., Dev Biol 2016 (PMID:27016259)
Kuo HY et al., Glycobiology 2016 (PMID:26447186)
Lin YC et al., J Leukoc Biol 2015 (PMID:25605870)
Yang JH et al., Nucleic Acids Res 2014 (PMID:25104022)
Chromatin Immunoprecipitation Assays for Myc and N-Myc
Myc and N-Myc have widespread impacts on the chromatin state within cells, both in a gene-specific and genome-wide manner. Our laboratory uses functional genomic methods including chromatin immunoprecipitation (ChIP), ChIP-chip
大阪大学蛋白质研究所藤井勇树、高木淳一东北大学大学院医学系研究科金子美华、加藤幸成 前言在分析蛋白质的功能时最重要的是如何得到高纯度的蛋白。现今提纯蛋白最常用的方法是亲和标签系统。被统称为「Epitope Tag」的肽标签以及其单克隆抗体组成的系统有FLAG、HA、Myc等多种标签。但是,每个标签系统都有优缺点,并不存在完美的标签系统。于是,在克服了众多难关后我们终于开发出有超高亲和性的新型亲和标签系统“PA Tag System”1)。 PA tag的起源Epitope Tag
Localization of the myc-Tagged 5HT5A Receptor by Immunogold Staining of Ultrathin Sections
of a specific protein occurs by a sandwich approach, consisting of a specific primary antibody directed against the target protein and a secondary antibody directed against the primary antibody, which is coupled to a marker for subsequent visualization
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