Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm in rat prostate by immunohistochemical analysis.
Sample: Paraffin-embedded rat prostate.
Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Grp78 antibody [N2C1], Internal detects Grp78 protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Grp78 antibody [N2C1], Internal (GTX102580) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
Immunoprecipitation of Grp78 protein from HepG2 whole cell extracts using 5 μg of Grp78 antibody [N2C1], Internal (GTX102580).
Western blot analysis was performed using Grp78 antibody [N2C1], Internal (GTX102580).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.Grp78 stained by Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.Grp78 stained by Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Grp78 antibody [N2C1], Internal detects Grp78 protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Grp78 antibody [N2C1], Internal (GTX102580) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [N2C1], Internal (GTX102580) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.