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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 保质期:
1年
- 英文名:
Di-ubiquitin [Ub2] (K33-linked) (human) (rec.)
- 库存:
大量
- 供应商:
欣博盛
- 规格:
25 ug
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文献和实验Parkinsons Disease: Assays for the Ubiquitin Ligase Activity of Neural Parkin
ubiquitin protein ligase activity to wild-type (but not mutant) Parkin proteins. Although the individual components of the proposed Parkin ubiquitin ligase complex in the normal human brain remain to be identified and the E3 ligase effect of Parkin function
ELISPOT (Enzyme-linked ImmunoSPOT)
Introduction Enzyme-linked immunosorbent spot (ELISPOT) assays were originally developed to enumerate B cells secreting antigen-specific antibodies, but subsequently the assay has been adapted for identification and enumeration
Purification of E1 and E1-Like Enzymes
physical and mechanistic features of the E1 family appear well conserved. Formation of an obligatory E1-ubiquitin thiol ester intermediate forms the basis of a one-step covalent purification of the enzyme on ubiquitin-linked affinity columns
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