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- 详细信息
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- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Rho Family GTPase 1 (RND1)
- 库存：
1000
- 供应商：
上海信裕
Rho家族GTP酶1(RND1)重组蛋白
Recombinant Rho Family GTPase 1 (RND1)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 90% |
| UOM | 50ug |
| Predicted Molecular Mass | 29.4kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Met1~Cys229 with two N-terminal Tags, His-tag and T7-tag |
Rho家族GTP酶1(RND1)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The sequence of the target protein is listed below.
MKERRAPQPV VARCKLVLVG DVQCGKTAML QVLAKDCYPE TYVPTVFENY TACLETEEQR VELSLWDTSG SPYYDNVRPL CYSDSDAVLL CFDISRPETV DSALKKWRTE ILDYCPSTRV LLIGCKTDLR TDLSTLMELS HQKQAPISYE QGCAIAKQLG AEIYLEGSAF TSEKSIHSIF RTASMLCLNK PSPLPQKSPV RSLSKRLLHL PSRSELISST FKKEKAKSC
MKERRAPQPV VARCKLVLVG DVQCGKTAML QVLAKDCYPE TYVPTVFENY TACLETEEQR VELSLWDTSG SPYYDNVRPL CYSDSDAVLL CFDISRPETV DSALKKWRTE ILDYCPSTRV LLIGCKTDLR TDLSTLMELS HQKQAPISYE QGCAIAKQLG AEIYLEGSAF TSEKSIHSIF RTASMLCLNK PSPLPQKSPV RSLSKRLLHL PSRSELISST FKKEKAKSC
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Rho家族GTP酶1(RND1)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Rho家族GTP酶1(RND1)重组蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
肝炎病毒包膜E2蛋白相同的亲和结合力3。另外，有阴离子转运载体AE1结构域的重组蛋白被标记且用Rho1D4系统纯化后表现出与红细胞来源的AE1蛋白有相同的硫酸外排速率。4） 利用Rho1D4纯化膜蛋白图解简述步骤1：结合：将Rho1D4标记的蛋白与载有Rho1D4抗体的琼脂糖一同孵育使得目标蛋白被固定在吸附材料上。 步骤2：清洗：将杂蛋白和其他裂解液成分洗去，留下与亲和基质结合的目标蛋白。 步骤3：洗脱：过量Rho1D4肽与基质竞争性结合，释放出目标蛋白在洗脱液中收集。 表
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Rho家族GTP酶1(RND1)重组蛋白
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