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文献和实验Protocol for First-strand cDNA Synth
Prepare in a sterile tube: template RNA: total RNA 0.1-5µg or poly(A)+ mRNA 10ng-0.5µg, or specific RNA 0.01pg-0.5µg primer: oligo(dT)18 0.5µg
How Do I make an Antibody against a Peptides
of mcKLH is highly soluble, making coupling reactions easier to complete. Figure 1. Peptide Carrier Conjugation using Maleimide Activated Carriers Figure 2. EDC-Mediated Peptide-Carrier Conjugation
Too many bands on a Western blot
Too many bands on a Western blot Western- or immunoblotting is a commonly employed technique for the detection of protein antigens in complex mixtures. Samples are first separated by SDS-polyacrylamide gel
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