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- 2026年04月21日
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- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Polymerase DNA Directed Alpha 1 (POLa1)
- 库存：
1000
- 供应商：
上海信裕
| Organism species | Mus musculus (Mouse) |
| Product No. | |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 33.5kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Ala1209~Ser1465 with two N-terminal Tags, His-tag and T7-tag |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
AI DTQYYLAQQI HPVVARICEP IDGIDAVLIA LWLGLDSTQF RVHQYHKDEE NDALLGGPAQ LTDEEKYKDC EKFKCLCPSC GTENIYDNVF EGSGLDMEPS LYRCSNVDCK VSPLTFMVQL SNKLIMDIRR CIKKYYDGWL ICEEPTCCSR LRRLPLHFSR NGPLCPVCMK AVLRPEYSDK SLYTQLCFYR YIFDADCALE KLTEHEKDKL KKQFFPLRVL QDYRKVKNIA EQFLSWSGYS EVNLSKLFAN YAGKS
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验The α1B -adrenergic receptor (α1B -AR) is a G-protein-coupled receptor that plays a key role in the sympathetic regulation of a variety of physiological processes, such as cardiac and smooth muscle contractility, contraction of the spleen, liver
佚名 1957年，Arthur kornberg首次在大肠杆菌中发现DNA聚合酶Ⅰ，(DNa polymerase Ⅰ,简写DNA polⅠ)后来又相继发现了DNA聚合酶Ⅱ和DNA聚合酶Ⅲ。(DNa polymerase Ⅱ，Ⅲ，简写DNA polⅡ,DNA polⅢ)实验证明大肠杆菌中DNA复制的主要过程靠DNa polⅢ起作用，而DNA polⅠ和DNA polⅡ在DNA
在细胞中存在的数目不多，是促进DNA链延长的主要酶。 发现 在50年代的中期，A. Kornberg和他的同事们就想到DNA的复制必然是一种酶的催化作用，于是决心分离出这种酶并研究其结构和作用机制。为了达到这个目的，他们分离的蛋白，然后加到体外合成系统中即同位素标记的dNTP、Mg2+及模板DNA，经过大量的工作，于1956年终于发现了DNA聚合酶Ⅰ(DNA polymerase Ⅰ，DNA pol Ⅰ)原来称为Kornberg酶。以后又相续发现了DNA pol Ⅱ和DNA
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