Against human IgG and human serum prior to conjugation
Antibody Form
Whole Antibody
Tested Applications
Dilution *
Flow Cytometry (Flow)
1-10 µg/mL
Immunocytochemistry (ICC)
1-10 µg/mL
Immunofluorescence (IF)
1-10 µg/mL
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Form information
Form
Liquid
Concentration
2 mg/ml
Purification
purified
Storage buffer
PBS, pH 7.5
Preservative
5mM sodium azide
Storage Conditions
4° C, store in dark
Gene/Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.