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- 加拿大DLDEVELOP
- 详见说明书
- 加拿大
- DL-BMP2-P
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量供应
- 供应商：
康朗生物
- 检测范围：
详见说明书
- 检测方法：
夹心法
- 应用：
ELISA 定量检测
- 标记物：
详见说明书
- 样本：
血清，血浆，尿液 细胞裂解液，组织匀浆，细胞培养上清液
- 规格：
96T/盒
Porcine Bone Morphogenetic Protein 2 (BMP2) ELISA Kit
| Product name: | Porcine Bone Morphogenetic Protein 2 (BMP2) ELISA Kit |
| Method: | sandwich |
| Synonyms: | |
| Catalog number: | DL-BMP2-P |
| Detection range: | 31.2-2,000pg/mL |
| Size: | 96T |
| Assay length | 1-4.5Hours |
| Price: | inquiry |
| Quality guarantee period: | for 12 months |
Introduction
Porcine Bone Morphogenetic Protein 2 (BMP2) ELISA Kit
| Item | Standard | Test Result | |
| Description | This immunoassay kit allows for the specific measurement of this index in serum, Plasma , Urine ,tissue homogenates and Cell culture supernates and Other biological fluids.. |
Conform | |
| Identification | Colorimetric | Positive | |
| Composition | Pre-coated, ready to use 96-well strip plate Standard (freeze dried) Standard Diluent Detection Reagent A Detection Reagent B Assay Diluent A Assay Diluent B TMB Substhumane Stop Solution Wash Buffer(30 x concenthumane) Plate sealer for 96 wells Instruction manual |
1 2 1 × 20ml 1× 120μl 1× 120μl 1 × 12ml 1 × 12ml 1 × 9ml 1 ×6ml 1 ×20ml 2 1 |
Conform |
Porcine Bone Morphogenetic Protein 2 (BMP2) ELISA Kit
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to this index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for this index and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve
Recovery
Matrices listed below were spiked with certain level of recombinant the index and the recovery humanes were calculated by comparing the measured value to the expected amount of the index in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 81-93 | 86 |
| EDTA plasma(n=5) | 80-97 | 88 |
| heparin plasma(n=5) | 90-101 | 95 |
Porcine Bone Morphogenetic Protein 2 (BMP2) ELISA Kit
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concenthumanion of the index and their serial dilutions. The results were demonsthumaned by the percentage of calculated concenthumanion to the expected.
| Sample | 1：2 | 1：4 | 1：8 | 1：16 |
| serum(n=5) | 82-96% | 83-98% | 81-99% | 93-101% |
| EDTA plasma(n=5) | 88-101% | 86-95% | 90-102% | 80-93% |
| heparin plasma(n=5) | 80-91% | 82-90% | 95-104% | 79-95% |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37℃;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
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文献和实验-β)1、2、3，骨形态发生蛋白(bone morphogenetic protein，BMP)2、6、9、13，胰岛素样生长因子-1(insulin-like growth factor-1，IGF-1)，软骨源性发生蛋白cartilage-derived morphogenetic protein，CDMP)1、2及地塞米松等。TGF-β主要作用于诱导的早期阶段，一般认为通过其受体激活Smad传导通路而启动软骨特异性基因的转录，主要是上调Ⅱ型胶原的表达与合成。BMP2、6等则可明显增强聚集蛋白
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
) Materials Osteocalcin sandwich ELISA kit (Biomedical Technologies, cat. no. BT‐470) including:
cells/mL) were incubated with LPS (2.5 μg/mL) in the presence or absence of hydroquinone for 6 hours. Supernatants were assayed for the levels of TNF-α, IL-1β, and IL-6 using a mouse TNF-α ELISA kit (Amersham, Little Chalfont, Buckinghamshire, UK).2
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