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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant GLI Family Zinc Finger Protein 1 (GLI1)
- 库存:
1000
- 供应商:
上海信裕
GLI家族锌指蛋白1(GLI1)重组蛋白
Recombinant GLI Family Zinc Finger Protein 1 (GLI1)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Rattus norvegicus (Rat) |
| Product No. | |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 35.0kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Val810~Ala1109 with two N-terminal Tags, His-tag and T7-tag |
GLI家族锌指蛋白1(GLI1)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The sequence of the target protein is listed below.
V QVKPEQGCPV GSDSTGLAPC LNAHPSEGSP DPQPLFSHHS QLPPPQYLQS GPYPQPPHDC LSLESRPGLN FNPSSHSTGQ LKAQLLCNYV QSQQELLWEG RSRGGIPNQE LPYQSPKFLG GSQVSQSPTK VPAAAAAATF GSGFASPALA NHKSVSYPAP SPCHETFTMG VNRPPHRPAA PPRLLPPLPP CYGPLKVGDA NPSCGHPEVG RLGAGPALYP PPEGQVCNPL DSLDLDNTQL DFVAILDEAQ GLSPPPSHEQ GDSSKNTPPP SGPPNMAVGN MSVLLGSLPG ETQFLNSSA
V QVKPEQGCPV GSDSTGLAPC LNAHPSEGSP DPQPLFSHHS QLPPPQYLQS GPYPQPPHDC LSLESRPGLN FNPSSHSTGQ LKAQLLCNYV QSQQELLWEG RSRGGIPNQE LPYQSPKFLG GSQVSQSPTK VPAAAAAATF GSGFASPALA NHKSVSYPAP SPCHETFTMG VNRPPHRPAA PPRLLPPLPP CYGPLKVGDA NPSCGHPEVG RLGAGPALYP PPEGQVCNPL DSLDLDNTQL DFVAILDEAQ GLSPPPSHEQ GDSSKNTPPP SGPPNMAVGN MSVLLGSLPG ETQFLNSSA
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
GLI家族锌指蛋白1(GLI1)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
GLI家族锌指蛋白1(GLI1)重组蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
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GLI家族锌指蛋白1(GLI1)重组蛋白
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