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- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Feline Sarcoma Oncogene (FES)
- 库存：
1000
- 供应商：
上海信裕
猫科肉瘤癌基因(FES)重组蛋白
Recombinant Feline Sarcoma Oncogene (FES)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 32.6kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Leu509~Asn766 (Accession # P07332) with two N-terminal Tags, His-tag and T7-tag |
猫科肉瘤癌基因(FES)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The sequence of the target protein is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LD NLYRLEGEGF PSIPLLIDHL LSTQQPLTKK SGVVLHRAVP KDKWVLNHED LVLGEQIGRG NFGEVFSGRL RADNTLVAVK SCRETLPPDL KAKFLQEARI LKQYSHPNIV RLIGVCTQKQ PIYIVMELVQ GGDFLTFLRT EGARLRVKTL LQMVGDAAAG MEYLESKCCI HRDLAARNCL VTEKNVLKIS DFGMSREEAD GVYAASGGLR QVPVKWTAPE ALNYGRYSSE SDVWSFGILL WETFSLGASP YPNLSN
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LD NLYRLEGEGF PSIPLLIDHL LSTQQPLTKK SGVVLHRAVP KDKWVLNHED LVLGEQIGRG NFGEVFSGRL RADNTLVAVK SCRETLPPDL KAKFLQEARI LKQYSHPNIV RLIGVCTQKQ PIYIVMELVQ GGDFLTFLRT EGARLRVKTL LQMVGDAAAG MEYLESKCCI HRDLAARNCL VTEKNVLKIS DFGMSREEAD GVYAASGGLR QVPVKWTAPE ALNYGRYSSE SDVWSFGILL WETFSLGASP YPNLSN
USAGE
猫科肉瘤癌基因(FES)重组蛋白Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
猫科肉瘤癌基因(FES)重组蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
发生率增多。然而，其肿瘤类型仅为淋巴网状肿瘤（网状细胞肉瘤、淋巴肉瘤），其他肿瘤的发生率并无明显增高。至今人们对该学说仍有争议，肿瘤的免疫监视学说还有待进一步证实。七十年代层掀起一次肿瘤免疫治疗高潮，是以非特异性免疫治疗为主。最具代表性的是采用细菌制剂，如：卡介苗（BCG）、短小棒状杆菌（C. Pavum）。经过几年的研究，除了用BCG膀胱灌注治疗膀胱癌获得明显疗效外，人们对它们逐渐失去了兴趣，因为对其他肿瘤远期疗效并未显著提高，甚至有些结果还不如对照组。同时因未找到人类肿瘤特异性抗原，人们对免疫
组分包括以下几大类物质： (1）促贴壁物质：许多细胞必须贴壁才能生长，这种情况下无血清培养基中一定要添加促贴壁和扩展因子，一般为细胞外基质，如纤连蛋白、层粘连蛋白等。它们还是重要的分裂素以及维持正常细胞功能的分化因子，对许多细胞的繁殖和分化，起着重要作用。纤连蛋白主要促进来自中胚层细胞的贴壁与分化，这些细胞包括成纤维细胞、肉瘤细胞、粒细胞、肾上皮细胞、肾上腺皮质细胞、CHO细胞、成肌细胞等。 (2）促生长因子及激素：针对不同细胞添加不同的生长因子。激素也是刺激细胞生长、维持细胞功能的重要物质
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猫科肉瘤癌基因(FES)重组蛋白
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