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- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
心型脂肪酸结合蛋白(FABP3)重组蛋白
- 库存：
1000
- 供应商：
上海信裕
心型脂肪酸结合蛋白(FABP3)重组蛋白
Recombinant Fatty Acid Binding Protein 3, Muscle And Heart (FABP3)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Bos taurus; Bovine (Cattle) |
| Product No. | |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 18.3kDa |
| Concentration | 8.7 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Met1~Ala133 (Accession # P10790) with two N-terminal Tags, His-tag and T7-tag |
心型脂肪酸结合蛋白(FABP3)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The sequence of the target protein is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- MVDAFVGTWK LVDSKNFDDY MKSLGVGFAT RQVGNMTKPT TIIEVNGDTV IIKTQSTFKN TEISFKLGVE FDETTADDRK VKSIVTLDGG KLVHVQKWNG QETSLVREMV DGKLILTLTH GTAVCTRTYE KQA
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- MVDAFVGTWK LVDSKNFDDY MKSLGVGFAT RQVGNMTKPT TIIEVNGDTV IIKTQSTFKN TEISFKLGVE FDETTADDRK VKSIVTLDGG KLVHVQKWNG QETSLVREMV DGKLILTLTH GTAVCTRTYE KQA
USAGE
心型脂肪酸结合蛋白(FABP3)重组蛋白Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
心型脂肪酸结合蛋白(FABP3)重组蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
的全新分子机制。研究发现，肥胖时脂肪细胞分泌的脂肪细胞脂肪酸结合蛋白（AFABP/FABP4）会结合血浆转铁蛋白（Transferrin, TSF），干扰铁转运功能，导致血清非转铁蛋白结合铁（NTBI）升高，引发肠干细胞铁过载。铁过载抑制PPARα信号，导致损伤后过氧化物酶体功能下降，进一步扰乱Wnt/Notch分化相关信号平衡，最终阻断成熟上皮分化，拖慢肠道修复。研究团队通过Cre依赖AAV在脂肪组织中定点提高AFABP，直接在瘦鼠身上复现了相关表型，为"脂肪-肠道"器官间通讯提供了强有力的因果
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心型脂肪酸结合蛋白(FABP3)重组蛋白
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