Recombinant Chimeric Chagas Mu

Production of Chimeric Heavy-Chain Antibodies

-chain antibodies (HCAbs) provide an excellent building block for constructing antibodies with moderate size yet with an intact Fc. We describe in this chapter the construction, production, and purification of chimeric HCAbs (cHCAbs), that is, fusion

Creation of Chimeric Junctions, Deletions, and Insertions by PCR

-directed mutagenesis, and construction of chimeric molecules at any chosen location in the molecule of interest (see Note 1 ). This chapter describes in detail a simplification of the original recombinant PCR method. This fast and efficient method

Expression of Chimeric Immunoglobulin Genes in Mammalian Cells

The ability to make monoclonal rodent antibodies has revolutionized immunology. To reduce the immunogenicity of these antibodies for human in vivo use, methods have been developed to create artificial recombinant antibodies (1 ). Chimeric