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文献和实验药品试剂:经BamHI 及HindIII 作用的pBlueGus 及pQE31含EtBr 的1.2% 12-well agarose gel1×TAE 电泳缓冲液QIAquick Gel Extraction Kit (Qiagen),内含:QIAquick spin column (管柱底部为silica gel membrane)Buffer QGBuffer PEBuffer EB (10 mM Tris-HCl, pH 8.5)2 mL 收集管TE-8.0方法步骤:1) 经BamHI
and eluted from silica with 30μl 1X restriction buffer •Procedure useful for cleaning DNA from PCR reactions,restriction digests,etc.as well as purifying from agarose gel slices •If 6 M NaI solution becomes yellow,it will still bind DNA if 1/200 volume 10
Fluorescent Silica Nanoparticles for Cancer Imaging
during the synthesis process. The synthetic process involves hydrolysis and condensation reactions of silane precursors. St�ber’s sol–gel and water-in-oil (W/O) microemulsion methods are two popular chemical methods that have been used for synthesizing FSNPs. Silica
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