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- DH220-2
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- 2025年07月15日
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文献和实验in 100 mM Tris-CL [pH 7.5], 0.9% NaCl) for two rinses of 15 min. each. The blot may be marked (with pencil or India Ink) for identification at this stage if desired.3. Block the blot with 10% nonfat dried milk (NFDM) freshly made in TTBS; rock
). Briefly: immerse in 50ml of BN buffer (0.1 M sodium bicarbonate, 0.1 % Nonidet P-40) for 10 minutes immerse in 50 ml of blocking buffer (5% nonfat milk in BN buffer) for 10 minutes immerse in 50 ml of anti-digoxigenin-fluorescein
Western Immunoblotting of Proteins
out at any step through development as this will cause an increase in background staining.] 3. Block the blotted nitrocellulose membrane in freshly prepared PBS containing 3% nonfat dry milk for 20 minutes at room temperature with constant agitation. A maximum
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