- ￥1384.50
- Abd Serotec
- 英国
- VMA00003KT
- 2026年01月23日
- WB
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液体
- 保存条件：
4度
- 克隆性：
PrecisionAb™ Monoclonal
- 标记物：
Purified
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
WB
- 浓度：
请参考说明书
- 靶点：
BETA 2 MICROGLOBULIN
- 抗体英文名：
BETA 2 MICROGLOBULIN ANTIBODY WITH CONTROL LYSATE
- 抗体名：
BETA 2 MICROGLOBULIN ANTIBODY WITH CONTROL LYSATE
- 规格：
2 Westerns
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文献和实验v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
for 1 min, discard the supernatant,and wash the beads two times in ice-cold PBS toremove unbound antibody. Resuspend the beads in 250 μL ofice-cold PBS. 3. Incubate 100 μL of beads with 400 μg of lysate in a totalvolume of 1 mL of cell lysis buffer (4°C
to include a "no antibody" control sample. Adjust the final volume of each sample with lysis buffer if required. Sample volumes should be between 400-800µl/sample to allow ample mixing during the overnight incubation. Add 1µg of antibody to each sample
Studies of the Ubiquitin Proteasome System
antibody for the protein of interest and isotype‐matched control antibody, e.g., irrelevant or preimmune serum, purified Tris‐buffered saline/Tween 20 (TBST; see recipe ), 4°C Cells growing in culture
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