手机验证
-20
The Liberase™ Research Grade Selection Kit provides small quantities of Liberase Research Grade Enzyme Blends for determining the optimal product for your application.
现货
Maximize viability and yield of isolated cells with an enzyme blend that has less clostripain and trypsin activity, as well as reduced endotoxin content.
5 × 5 mg
The Liberase™ Research Grade Selection Kit provides small quantities of Liberase Research Grade Enzyme Blends for determining the optimal product for your application.
• Maximize viability and yield of isolated cells
with an enzyme blend that has less clostripain and trypsin activity, as well as reduced endotoxin content.
• Count on higher specific activity of the enzyme blend
as a result of higher Collagenase I + II purity (determined by HPLC analysis).
• Obtain higher experimental reproducibility
due to higher lot-to-lot consistency.
• Increase safety
with an enzyme that is free of any mammalian or avian tissue-derived raw materials.
The Liberase Research Grade Selection Kit contains small quantities of Liberase DL Research Grade, Liberase DH Research Grade, Liberase TL Research Grade, Liberase TM Research Grade, and Liberase TH Research Grade; each quantity is provided in a separate vial.
The most commonly used method for decellularization procedures, such as tissue dissociation and cell harvesting, is based on the use of proteolytic enzymes. Proteases disrupt the extracellular matrix of tissue to allow the release of individual cells or the harvesting of cultured cells for transfer. The goal of cell isolation is to maximize the yield of dissociated cells that are viable and functionally active.
Liberase Purified Enzyme Blends
Liberase enzymes are blends of highly purified enzymes, designed to increase the quality and reproducibility of tissue dissociation, and improve the viability and functionality of isolated cells. Liberase Purified Enzyme Blends replace traditional collagenase, which is a crude and variable fermentation by-product of Clostridium histolyticum.
Liberase Enzyme Technology
Liberase enzyme technology comprises methods for purifying clostridial collagenase isoforms to high specific activity, and for blending them together with high-specific-activity neutral protease in optimal ratios for effective dissociation of primary tissues and cultured cells.
For additional information, such as manufacturing, technology, or storage, see the Liberase Research Grade Insights document.Further reading on the topic can also be found in the Documents tab.
Roche provides special-quality preparations of Liberase Purified Enzyme Blends, manufactured to meet GMP requirements.
For life science research only. Not for use in diagnostic procedures.
• This product is intended for life science research and in vitro use only. These products are not to be used for diagnostic or clinical applications, such as human islet transplantation.
• All 1st generation Liberase and Liberase Blendzyme products (Liberase HI, CI, RI, PI, Liberase Blendzyme 1, 2, 3, 4) have been replaced by the 2nd generation Liberase Research Grade and Liberase GMP grade enzyme blend portfolios. For instructions concerning the transition from previously used Liberase Enzymes.
1 kit containing 5 components.
Stabilizers: Calcium
Storage conditions (working solution): Store unused stock solution in single-use aliquots at -15 to -25 °C. For further information on product stability, please visit the Roche Liberase Enzyme website at www.collagenase.com.
Note: Avoid repeated freezing and thawing!
Reconstitute the lyophilized enzyme with injection-quality sterile water or tissue-dissociation buffer. Do not add serum or other components, such as albumin or protease inhibitors to the dissociation buffer. Enzyme stability is reduced at higher concentrations and warmer temperatures (4 °C). Avoid both the above conditions.
Reconstitute the entire vial. Do not weigh individual aliquots of the lyophilizate. The introduction of moisture into the vial will result in a decline in enzymatic activity.
Place vial on ice to rehydrate the lyophilized enzyme.
Gently agitate the vial at 2 to 8 °C, until the enzyme is completely dissolved (max. 30 min).
Depending on the type of tissue-dissociation buffer used to dissolve Liberase Research Grade Purified Enzyme Blends, slight precipitations may be observed which readily dissolve in the diluted working solution, and have no influence on enzyme activity.
Remove an aliquot of the stock solution to prepare the working solution.
Reconstitution volume
2 ml (1 vial with 5 mg–10 mg pack size)
10 ml (1 vial with 50 mg–100 mg pack size)
Collagenase Wünsch (units/ml)
13 (1 vial with 5 mg–10 mg pack size)
26 (1 vial with 50 mg–100 mg pack size)
Total Collagenase concentration [mg/ml]
2.5 (1 vial with 5 mg–10 mg pack size)
5.0 (1 vial with 50 mg–100 mg pack size)
Liberase is a trademark of Roche
packaging | kit of 5 × 5 mg |
mfr. no. | Roche |
limit | 35-37 °C optimum reaction temp. |
optimum pH | 7.4 |
shipped in | dry ice |
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。