产品名称
Frequently Asked QuestionsLive Chat and Frequently Asked Questions are available for this Product.包装Package size based on protein content.ReconstitutionWhen reconstituted at 1 mg/mL in water, solution will contain 0.05 M Tris buffer salts, pH 7.5, 0.2 M NaCl, 0.003 M NaN3, and 0.001 M sodium EDTA. Store reconstituted solutions in the refrigerator.Unit DefinitionOne unit increases adenylate cyclase activity 6-fold, measured as [3H]-cAMP present, using approx. 70,000 CHO cells after incubation for 90 min at 37 °C with [3H]-adenine followed by a 15 min incubation with 1 mM 3-isobutyl-1-methylxanthine (IBMX).Physical formLyophilized powder containing Tris buffer salts, sodium chloride, sodium azide and sodium EDTABiochem/physiol ActionsToxin consisting of an A subunit (27 kDa) surrounded by five B subunits (approximately 12 kDa each), which attach the toxin to ganglioside GM1 on the cell surface. The A subunit catalyzes ADP-ribosylation of the α-subunit of the stimulatory G protein (Gαs), reducing GTPase activity and activating the α-subunit. This activation of Gαs leads to an increase in the activity of adenylyl cyclase, resulting in increased levels of cAMP. Also ADP-ribosylates transducin in the eye rod outer segments, inactivating its GTPase activity. Cholera toxin has also been reported to ADP-ribosylate tubulin. Shown to be a potent mucosal vaccine adjuvant, inducing T helper cell type 2 responses by inhibiting the production of interleukin-12.Features and BenefitsThis compound is a featured product for Cyclic Nucleotide research. Click hereto discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.
性质
Related CategoriesBacteria, Bioactive Small Molecule Alphabetical Index, C-CH, G Protein Function, G Proteins and Cyclic Nucleotides,
更多...Quality Level PREMIUMassay ~95% (SDS-PAGE)form lyophilized powdercomposition Protein, ~5% Lowry-TCAsolubility H2O: soluble10 m