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文献和实验One-Step Purification of Recombinant Proteins with the 6xHis Tag and Ni-NTA Resin
nickel ions, giving a highly selective interaction that allows purification of tagged proteins or protein complexes from 95% homogeneity in just one step (1 ,2 ). The tight association between the tag and the Ni-NTA resin allows contaminants
Resin Technologies: Construction and Staining of Resin TMAs
the issue of antigenicity, frozen tissue suffers from a lack of morphology. We have demonstrated that tissue microarrays constructed in glycol methacrylate resin, when combined with a cold acetone fixation step, have been able to combine the superior
them down and continue. These pellets do not hold together very well so tke care that they do not fall apart in the resin. 12). Leave in fresh propylene oxide for 20' then in propylene oxide/Spurr's resin (1:1 mix) overnight. Mixing during the incubation will help
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