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文献和实验Total Protein Extraction with TCA-Acetone
We describe a procedure allowing extraction of total proteins that performs efficiently with a large variety of plant tissues, based on simultaneous precipitation and denaturation with TCA and 2ME in cold acetone. We also describe protein
Isolation of Total RNA from Difficult Tissues
techniques to help overcome problems associated with isolating total RNA from difficult tissues. The tips provided are based on RNA isolation by a guanidinium thiocyanate/acid phenol:chloroform extraction method (e.g. Ambion"s ToTALLY RNA™ Kit). Note
The Extraction of Total RNA by the Detergent and Phenol Method
Successful extraction of RNA depends on the quantitative recovery of pure nucleic acids in an undegraded form. In practice, this means that a selective extraction process is required to remove all the unwanted cellular material in a manner
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