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文献和实验Protein Extraction From Mammalian Tissues
For Western blotting and coimmunoprecipitation (coIP), protein samples must be extracted from tissues. The protocol described in this chapter has been used to extract clock proteins from mammalian tissues as diverse as liver, kidney
实验试剂 1. Sterile deionized water (or TE buffer) 2. Absolute (or 95%) ethanol 实验设备 1. Protective eye-ware 实验步骤 1. MicroElute™ Gel Extraction Spin Protocol
A method is presented for the extraction of total protein from Arabidopsis thaliana tissue. The protocol was designed for the solubilization of a range of proteins and their efficient and quantitative recovery. It is especially compatible
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