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文献和实验The Binding of Protein-Ligands to Cell-Surface Receptors
their physiological properties. Additionally, abnormal responses owing to receptor or ligand mutation cannot be fully characterized without reliable analysis of the normal state. This chapter discusses some of the practical considerations for studying protein-protein
should be taken from the refrigerator at least a half hour before opening, and they should be left in a dessicator. Before opening any of the vials, be sure it is not at all cool to the touch. All of the cell isolation enzymes cited in this section
Isolation of Human Tumor-Associated Cell Surface Antigen-Binding scFvs
-based immunotherapy of malignant diseases. More recently, TAA have been identified by utilizing phage-display Ab libraries, which are a diverse collection of Abs displayed as Fabs (4 ,5 ) or scFvs (6 ) on the surface of filamentous phages (for review
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